Impaired climbing and flight behaviour in Drosophila melanogaster following carbon dioxide anaesthesia
Nathan R. Bartholomew, Jacob
M. Burdett, John M. VandenBrooks, Michael C. Quinlan & Gerald B. Call
Scientific Reports 5,
Article number: 15298 (2015)
doi:10.1038/srep15298
Received:
10
May 2015
Accepted:
22
September 2015
Published
online:
19
October 2015
Abstract
Laboratories
that study Drosophila melanogaster or other insects commonly use carbon
dioxide (CO2) anaesthesia for sorting or other work. Unfortunately, the use of
CO2 has potential unwanted physiological effects, including altered respiratory
and muscle physiology, which impact motor function behaviours. The effects of
CO2 at different levels and exposure times were examined on the subsequent
recovery of motor function as assessed by climbing and flight assays. With as
little as a five minute exposure to 100% CO2, D. melanogaster exhibited
climbing deficits up to 24 hours after exposure. Any exposure length over five
minutes produced climbing deficits that lasted for days. Flight behaviour was
also impaired following CO2 exposure. Overall, there was a positive correlation
between CO2 exposure length and recovery time for both behaviours. Furthermore,
exposure to as little as 65% CO2 affected the motor capability of D.
melanogaster. These negative effects are due to both a CO2-specific
mechanism and an anoxic effect. These results indicate a heretofore
unconsidered impact of CO2 anaesthesia on subsequent behavioural tests
revealing the importance of monitoring and accounting for CO2 exposure when
performing physiological or behavioural studies in insects.
If you click on the names in the link it tells about them. Jacob and Bartholomew have Arizona Osteopathic Medicine AZCOM. Vanderbrooks and Quinlan are from the Arizona college
of Physiology and Call is from the Arizona college of Pharmacology.
Jacob says that "Vanderbrooks, Call and the other guy" have PhD's or are doctors. I am guessing the other guy is Quinlan.
Jacob's First Published article- he did the lab work.
If you click on the names in the link it tells about them. Jacob and Bartholomew have Arizona Osteopathic Medicine AZCOM. Vanderbrooks and Quinlan are from the Arizona college
of Physiology and Call is from the Arizona college of Pharmacology.
Jacob says that "Vanderbrooks, Call and the other guy" have PhD's or are doctors. I am guessing the other guy is Quinlan.
Jacob's First Published article- he did the lab work.
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